Designing reproducible PCR assays involves optimizing multiple moving targets, from standardizing each component in sometimes minute reaction volumes to planning ahead to ensure long-term and secure ...

The key to an optimal PCR experiment is primer design. In general, primers should be between 18 and 24 nucleotides long and have a GC content between 40 and 60 percent. Ideally primers should contain ...

This webinar provides a practical introduction to the fundamentals of assay design for quantitative real-time PCR (qPCR), with a strong focus on optimizing specificity and performance. Attendees will ...

A new discovery has reshaped our understanding of PCR chip technology: water vapor, not air expansion, is the primary culprit behind bubble formation in polydimethylsiloxane (PDMS)-based chips. This ...